2. Biosynthesis process of DNADNA replication system

★Template: double stranded DNA

★Substrate: dNTP

★Primer(引物): short RNA fragment with a free 3´-OH end

★Enzyme : DNA-dependent DNA polymerase (DDDP)

                 Other enzyme and protein factors

2.1 Enzymology of DNA Replication

Enzyme : 

★Helicase (解螺旋酶)

★Topoisomerase（拓扑异构酶）

★SSB（单链DNA结合蛋白）

★Primase（引物酶）

★DNA-dependent DNA polymerase (DDDP)（DNA聚合酶）

★Ligase（连接酶）

★protein factor（蛋白因子）

2.1.1 Helicase

★It is also referred to as 解螺旋酶/解链酶 with ATPase activity(ATP酶活性)

★DnaB is main enzyme in E.coli DNA replication.

★It unwinds the double strand DNA and help primase to get access to DNA template.

★The opening process with the assistance of DnaA and DnaC

★In eukaryote: MCM