2.3  Protein synthesis in eukaryote 
                — different from prokaryote
                真核生物蛋白质的生物合成

需要更多因子和步骤

★1.翻译和转录过程不偶联

★2.5‘ cap 和3’ poly(A) tail 参与蛋白翻译的起始.

★3.在mRNA无SD 序列

★4. Ribosome

1. Prokaryote:translation is coupledwith transcription

★Eukaryote: uncoupled gene expression

2. 原核生物的多顺反子 (Polycistron)

3.  Biologicalsynthesis process

★ A. initation

 · (1) Morefactors

 · (2)Different binding sequence

 ·elF4E: Cap binding protein

            ( 帽结合蛋白)

 ·PAB: Poly A tail binding protein

        （poly A尾结合蛋白）

  ★ B. Elongation in eukaryote

 ·Similar to prokaryote but with different factors

 ·EF-Tu—— eEF1α

 ·EF-Ts  ——eEF1βγ

 ·EF-G   ——eEF-2

 ·There is no E site in eukaryote.

★ C.Termination in eukaryote：one RF

★4. Protein synthesisin mitochondria and chloroplast is similar to that in prokaryote.