知识要点key points
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【知识要点】如何获得阳性重组子----目的基因和载体的连接方式、连接结果?如何保证目的基因与载体正确连接? 如何筛选阳性重组子?
【 Key points 】 How to obtain positive recombinon ---- the method and result of the connection between the target gene and the vector? How to ensure the correct connection between target gene and vector? How to screen positive recombinants?
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